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Studies of the propagation and antibiotic sensitivity of C. trachomatis.

Mumtaz, Gilanfar; (1990) Studies of the propagation and antibiotic sensitivity of C. trachomatis. Doctoral thesis (M.Phil.), University College London. Green open access

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Chlamydiae, being intracellular parasites with a unique life cycle, evaded close laboratory scrutiny until 1932 when bedson and bland followed the life cycle of chlamydia psittaci in the tissues of dead animals. In 1957, successful isolation of chlamydia trachomatis in embryonated hens' eggs opened a whole new chapter in the elucidation of the life cycle of this organism. A part of the work presented in this thesis follows the life cycle of c.trachomatis using transmission electron microscopy, time lapse photography and a number of staining techniques suitable for light microscopy. The isolation of c.trachomatis in eggs by t'ang et al (1957) and later in tissue culture by gordon and quan (1965b) was a major technical advance in producing chlamydial antigen in bulk. An alternative, easier technique which gives both a large yield of c.trachomatis and saves the burden of inoculating eggs or several bottles of tissue culture, as is current practice, is presented in this thesis. This work involves the use of microcarriers which have elsewhere been used for growing viruses, cells and their products in bulk. During the last decade, c.trachomatis has been recognised as one of the most common sexually transmitted pathogens as well as the commonest cause of preventable blindness. In addition to uncomplicated infections, namely urethritis in both sexes, cervicitis in women and neonatal conjunctivitis, serious sequelae such as epididymitis, perihepatitis, pelvic imflammatory disease (with the attendant risk of infertility) and in infants a characteristic pneumonitis syndrome may result. Effective chemotherapy against chlamydial infection has been possible since the advent of sulphonamides, tetracyclines and erythromycin. Part of the work presented in this thesis examines the effect of over 40 different antimicrobial agents against c.trachomatis using a standard tissue culture technique.

Type: Thesis (Doctoral)
Qualification: M.Phil.
Title: Studies of the propagation and antibiotic sensitivity of C. trachomatis.
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis Digitised by Proquest.
URI: https://discovery.ucl.ac.uk/id/eprint/10124105
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