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Primary tissue culture of human laryngeal carcioma and interaction with native lymphocytes

Wang, Helen Sui Lan; (1990) Primary tissue culture of human laryngeal carcioma and interaction with native lymphocytes. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

Primary tissue culture of human laryngeal carcinomas, producing cells similar to the original tumour, was effected in serum-supplemented medium. This culture method provided a good investigative model for the study of lymphocyte/epithelial cell interactions in tumour and normal cultures. Of 107 laryngeal and hypopharyngeal carcinomas, 45% grew. The appearances were compared with similar preparations taken from normal true cord of the same larynx, of which 70% grew. No morphological differences were found by light or electron microscopy. Bizarre morphology occurred in 20% of cultures, mainly in tumour but also in normal cultures. This appeared in cultures which had ceased proliferation at an earlier stage than non-bizarre cultures and was identified by inactivity of monolayer and lack of intracellular activity, as observed by time-lapse video microscopy. Cultures were revealed by time-lapse video microscopy to support motile lymphocytes, which appeared phase-dark, both on the upper and lower monolayer surfaces under phase-contrast optics. Tumour-infiltrating lymphocytes migrated from laryngeal explants onto the emerging monolayer. These cells remained activated on the culture for as long as 19 days. Many attempts were made by tracking methods to identify lymphocyte phenotype, which was found to be T cell, although heterogeneous subset identities existed. Bizarre type cultures lacked motile lymphoid cells suggesting that lymphocytes require stimulants produced by proliferating cultured epithelial cells. Mitotic tumour cells were shown, using vector analysis, to be chemotactic for T cells. This was a unique function of the culture method. This chemotactic phenomenon could not be repeated if diffusion was increased, presumably because products of cell growth were dissipated. Mitotic chemotaxis be related to mediators by the epithelium specific for T cells.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: Primary tissue culture of human laryngeal carcioma and interaction with native lymphocytes
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: Health and environmental sciences; Laryngeal carcinomas
URI: https://discovery.ucl.ac.uk/id/eprint/10123538
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