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SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay [version 1; peer review: 1 approved with reservations]

Buck, M; Poirier, E; Cardoso, A; Frederico, B; Canton, J; Barrell, S; Beale, R; ... The Crick COVID-19 Consortium; + view all (2021) SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay [version 1; peer review: 1 approved with reservations]. Wellcome Open Research , 6 (9) 10.12688/wellcomeopenres.16517.1. Green open access

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Abstract

The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARSCoV-2 detection by RT-LAMP in 25 minutes that is robust, reliable, repeatable, sensitive, specific, and inexpensive.

Type: Article
Title: SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay [version 1; peer review: 1 approved with reservations]
Open access status: An open access version is available from UCL Discovery
DOI: 10.12688/wellcomeopenres.16517.1
Publisher version: http://doi.org/10.12688/wellcomeopenres.16517.1
Language: English
Additional information: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0/
Keywords: SARS-CoV-2, clinical diagnostic, RT-LAMP
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Renal Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10123088
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