Boakye, Pearl Angela Adwoa Serwah;
(1992)
The effect of oxygen and glucose availability on synaptosomal function.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
In this study, cerebral ischaemic damage was investigated at the cellular level using isolated nerve terminals, synaptosomes, prepared from adult rat brain. During ischaemia there is both a deficient supply of oxygen and substrate. The effect of altering individual and different combinations of the parameters involved in the ischaemic episode were investigated. A lack of oxygen supply (anoxia) and substrate (glucose) supply (aglycaemia) were assessed separately. Synaptosomes were also incubated in anoxic conditions exacerbated by low glucose availability as a model of ischaemic brain damage in vivo. The involvement, during ischaemia, of the cellular parameters of calcium homeostasis, lactosis, acidosis, membrane depolarisation and synaptic transmission was assessed by studying alterations in these parameters under conditions of different oxygen and substrate availability. Integrity of the synaptosomal plasma membrane was assessed by release of choline from membrane phospholipids. Calcium homeostasis was investigated by measuring calcium uptake and intrasynaptosomal calcium concentration ([ Ca++ ]i) . Anoxia resulted in an increased production of lactate and a decrease in [Ca++]i, calcium uptake and acetylcholine release. In contrast, ischaemia resulted in decreased lactate production but an increase in [Ca++]i, calcium uptake and acetylcholine release. A possible role for lactate in synaptosomal function was investigated by the addition of lactate to normoxic synaptosomes and this reproduced similar effects to those recorded in anoxia. It was also found that lactate could protect synaptosomes from the calcium overload induced by ischaemia. The results are discussed with respect to the possible role of lactate in synaptosomal function.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | The effect of oxygen and glucose availability on synaptosomal function |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Thesis digitised by ProQuest. |
| Keywords: | Biological sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10122321 |
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