UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

α-Helical Peptides on Plasma-Treated Polymers Promote Ciliation of Airway Epithelial Cells

Mehrban, N; Cardinale, D; Gallo, SC; Lee, DDH; Scott, DA; Dong, H; Bowen, J; ... Callaghan, CO; + view all (2021) α-Helical Peptides on Plasma-Treated Polymers Promote Ciliation of Airway Epithelial Cells. Materials Science and Engineering: C , Article 111935. 10.1016/j.msec.2021.111935. (In press).

[thumbnail of Mehrban_1-s2.0-S0928493121000734-main.pdf] Text
Mehrban_1-s2.0-S0928493121000734-main.pdf - Accepted version
Access restricted to UCL open access staff until 6 February 2022.

Download (2MB)

Abstract

Airway respiratory epithelium forms a physical barrier through intercellular tight junctions, which prevents debris from passing through to the internal environment while ciliated epithelial cells expel particulate-trapping mucus up the airway. Polymeric solutions to loss of airway structure and integrity have been unable to fully restore functional epithelium. We hypothesized that plasma treatment of polymers would permit adsorption of α-helical peptides and that this would promote functional differentiation of airway epithelial cells. Five candidate plasma compositions are compared; Air, N2, H2, H2:N2 and Air:N2. X-ray photoelectron spectroscopy shows changes in at% N and C 1s peaks after plasma treatment while electron microscopy indicates successful adsorption of hydrogelating self-assembling fibres (hSAF) on all samples. Subsequently, adsorbed hSAFs support human nasal epithelial cell attachment and proliferation and induce differentiation at an air-liquid interface. Transepithelial measurements show that the cells form tight junctions and produce cilia beating at the normal expected frequency of 10-11 Hz after 28 days in culture. The synthetic peptide system described in this study offers potential superiority as an epithelial regeneration substrate over present “gold-standard” materials, such as collagen, as they are controllable and can be chemically functionalised to support a variety of in vivo environments. Using the hSAF peptides described here in combination with plasma-treated polymeric surfaces could offer a way of improving the functionality and integration of implantable polymers for aerodigestive tract reconstruction and regeneration.

Type: Article
Title: α-Helical Peptides on Plasma-Treated Polymers Promote Ciliation of Airway Epithelial Cells
DOI: 10.1016/j.msec.2021.111935
Publisher version: https://doi.org/10.1016/j.msec.2021.111935
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Polymer, Peptide, Plasma Treatment, Cilia, Biomaterial, Airway
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > The Ear Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10121142
Downloads since deposit
1Download
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item