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Deciphering Organoids: High-Dimensional Analysis of Biomimetic Cultures

Qin, X; Tape, CJ; (2020) Deciphering Organoids: High-Dimensional Analysis of Biomimetic Cultures. Trends in Biotechnology , 39 (8) pp. 774-787. 10.1016/j.tibtech.2020.10.013. Green open access

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Abstract

Organoids are self-organising stem cell-derived ex vivo cultures widely adopted as biomimetic models of healthy and diseased tissues. Traditional low-dimensional experimental methods such as microscopy and bulk molecular analysis have generated remarkable biological insights from organoids. However, as complex heterocellular systems, organoids are especially well-positioned to take advantage of emerging high-dimensional technologies. In particular, single-cell methods offer considerable opportunities to analyse organoids at unprecedented scale and depth, enabling comprehensive characterisation of cellular processes and spatial organisation underpinning organoid heterogeneity. This review evaluates state-of-the-art analytical methods applied to organoids, discusses the latest advances in single-cell technologies, and speculates on the integration of these two rapidly developing fields.

Type: Article
Title: Deciphering Organoids: High-Dimensional Analysis of Biomimetic Cultures
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.tibtech.2020.10.013
Publisher version: https://doi.org/10.1016/j.tibtech.2020.10.013
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: omics technology, organoid, single-cell analysis, systems biology
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
URI: https://discovery.ucl.ac.uk/id/eprint/10120396
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