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B cells extract antigens at Arp2/3-generated actin foci interspersed with linear filaments

Roper, SI; Wasim, L; Malinova, D; Way, M; Cox, S; Tolar, P; (2019) B cells extract antigens at Arp2/3-generated actin foci interspersed with linear filaments. eLife , 8 , Article e48093. 10.7554/eLife.48093. Green open access

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Abstract

Antibody production depends on B cell internalization and presentation of antigens to helper T cells. To acquire antigens displayed by antigen-presenting cells, B cells form immune synapses and extract antigens by the mechanical activity of the acto-myosin cytoskeleton. While cytoskeleton organization driving the initial formation of the B cell synapse has been studied, how the cytoskeleton supports antigen extraction remains poorly understood. Here we show that after initial cell spreading, F-actin in synapses of primary mouse B cells and human B cell lines forms a highly dynamic pattern composed of actin foci interspersed with linear filaments and myosin IIa. The foci are generated by Arp2/3-mediated branched-actin polymerization and stochastically associate with antigen clusters to mediate internalization. However, antigen extraction also requires the activity of formins, which reside near the foci and produce the interspersed filaments. Thus, a cooperation of branched-actin foci supported by linear filaments underlies B cell mechanics during antigen extraction.

Type: Article
Title: B cells extract antigens at Arp2/3-generated actin foci interspersed with linear filaments
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.48093
Publisher version: https://doi.org/10.7554/eLife.48093
Language: English
Additional information: Copyright © 2019, Roper et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/10119748
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