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Stem Cell-Derived Respiratory Epithelial Cell Cultures as Human Disease Models

Orr, JC; Hynds, RE; (2021) Stem Cell-Derived Respiratory Epithelial Cell Cultures as Human Disease Models. American Journal of Respiratory Cell and Molecular Biology 10.1165/rcmb.2020-0440TR. (In press). Green open access

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Abstract

Advances in stem cell biology and the understanding of factors that determine lung stem cell self-renewal have enabled long-term in vitro culture of human lung cells derived from airway basal and alveolar type II cells. Improved capability to expand and study primary cells long-term, including in clonal cultures that are recently derived from a single cell, will allow experiments that address fundamental questions about lung homeostasis and repair, as well as translational questions in asthma, chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, and lung cancer research. Here, we provide a brief history of post-natal lung epithelial cell culture and describe recent methodological advances, including some culture systems that now permit clonal cell culture. We further discuss the applications of primary cultures in defining ‘normal’ epithelium, modelling lung disease and in future cell therapies.

Type: Article
Title: Stem Cell-Derived Respiratory Epithelial Cell Cultures as Human Disease Models
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1165/rcmb.2020-0440TR
Publisher version: https://doi.org/10.1165/rcmb.2020-0440TR
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: respiratory epithelium; epithelial cells; cell culture; cloning; stem cells
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10119335
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