Bennett, I;
Pyne, ALB;
McKendry, RA;
(2020)
Cantilever Sensors for Rapid Optical Antimicrobial Sensitivity Testing.
ACS Sensors
, 5
(10)
pp. 3133-3139.
10.1021/acssensors.0c01216.
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Abstract
Growing antimicrobial resistance (AMR) is a serious global threat to human health. Current methods to detect resistance include phenotypic antibiotic sensitivity testing (AST), which measures bacterial growth and is therefore hampered by a slow time to obtain results (∼12− 24 h). Therefore, new rapid phenotypic methods for AST are urgently needed. Nanomechanical cantilever sensors have recently shown promise for rapid AST but challenges of bacterial immobilization can lead to variable results. Herein, a novel cantilever-based method is described for detecting phenotypic antibiotic resistance within ∼45 min, capable of detecting single bacteria. This method does not require complex, variable bacterial immobilization and instead uses a laser and detector system to detect single bacterial cells in media as they pass through the laser focus. This provides a simple readout of bacterial antibiotic resistance by detecting growth (resistant) or death (sensitive), much faster than the current methods. The potential of this technique is demonstrated by determining the resistance in both laboratory and clinical strains of Escherichia coli (E. coli), a key species responsible for clinically burdensome urinary tract infections. This work provides the basis for a simple and fast diagnostic tool to detect antibiotic resistance in bacteria, reducing the health and economic burdens of AMR
Type: | Article |
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Title: | Cantilever Sensors for Rapid Optical Antimicrobial Sensitivity Testing |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1021/acssensors.0c01216 |
Publisher version: | https://doi.org/10.1021/acssensors.0c01216 |
Language: | English |
Additional information: | This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. http://creativecommons.org/licenses/by/4.0/ |
Keywords: | diagnostics, antimicrobial resistance, cantilevers, bacteria, antibiotics, optical |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
URI: | https://discovery.ucl.ac.uk/id/eprint/10119219 |
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