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Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method

Church, TW; Gold, MG; (2021) Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method. Patch Clamp Electrophysiology , 2188 pp. 243-257. 10.1007/978-1-0716-0818-0_12.

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Abstract

Cultured hippocampal slices from rodents, in which the architecture and functional properties of the hippocampal network are largely preserved, have proved to be a powerful substrate for studying healthy and pathological neuronal mechanisms. Here, we delineate the membrane-interface method for maintaining organotypic slices in culture for several weeks. The protocol includes procedures for dissecting hippocampus from rat brain, and collecting slices using a vibratome. This method provides the experimenter with easy access to both the brain tissue and culture medium, which facilitates genetic and pharmacological manipulations and enables experiments that incorporate imaging and electrophysiology. The method is generally applicable to rats of different ages, and to different brain regions, and can be modified for culture of slices from other species including mice.

Type: Article
Title: Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method
Location: United States
DOI: 10.1007/978-1-0716-0818-0_12
Publisher version: https://doi.org/10.1007/978-1-0716-0818-0_12
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
Keywords: Brain slice, Hippocampus, Organotypic, Whole-brain cultures
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/10119183
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