Cheong, Y-K;
Ren, GG;
Huang, M;
Detsch, R;
Boccaccini, AR;
(2017)
Biological evaluations of novel 2,3,3-Trisphosphonate in osteoclastic and osteoblastic activities.
General Medicine Open
, 2
(1)
10.15761/gmo.1000121.
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Abstract
Bisphosphonates (BPs) are the first line treatment for many bone diseases including hypercalcimia associated with bone malignancies. In this paper, we introduce a new analogue of bisphosphonate called the 2,3,3-Trisphosphonate (2,3,3-TriPP) that was synthesised in a two steps reaction. In vitro investigations using a medically known bisphosphonate (Etidronate) and the 2,3,3-TrisPP were performed with an aim to evaluate biological effect of this novel compound in major bone cells. 2,3,3-TrisPP showed to have potential to supress the bone resorption process, as our data found that this novel compound exhibited cytotoxic effect in osteoclastic cells at a low concentration of 0.172 mg/mL (LC50). A molecular docking computational simulation calculated a high level of binding affinity between the human farnesyl pyrophosphate synthase (hFPPS) and 2,3,3-TrisPP. This calculation suggested 2,3,3TrisPP may have undergone the mevalonate pathway to prevent the prenylation step during biosynthesis and subsequently resulted in the deactivation of osteoclastic cells. Finally, high levels of osteoblast mineralisation potentials were recorded upon treatments with 2,3,3-TrisPP (0.01-0.1 mg/ml), which implied 2,3,3-TrsiPP may also facilitate bone regeneration.
Type: | Article |
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Title: | Biological evaluations of novel 2,3,3-Trisphosphonate in osteoclastic and osteoblastic activities |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.15761/gmo.1000121 |
Publisher version: | http://dx.doi.org/10.15761/gmo.1000121 |
Language: | English |
Additional information: | © 2017 Cheong YK. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute |
URI: | https://discovery.ucl.ac.uk/id/eprint/10115382 |
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