Zhang, J-Y;
Wang, X-M;
Xing, X;
Xu, Z;
Zhang, C;
Song, J-W;
Fan, X;
... Wang, F-S; + view all
(2020)
Single-cell landscape of immunological responses in patients with COVID-19.
Nature Immunology
, 21
(9)
pp. 1107-1118.
10.1038/s41590-020-0762-x.
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Abstract
In coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, the relationship between disease severity and the host immune response is not fully understood. Here we performed single-cell RNA sequencing in peripheral blood samples of 5 healthy donors and 13 patients with COVID-19, including moderate, severe and convalescent cases. Through determining the transcriptional profiles of immune cells, coupled with assembled T cell receptor and B cell receptor sequences, we analyzed the functional properties of immune cells. Most cell types in patients with COVID-19 showed a strong interferon-α response and an overall acute inflammatory response. Moreover, intensive expansion of highly cytotoxic effector T cell subsets, such as CD4^{+} effector-GNLY (granulysin), CD8^{+} effector-GNLY and NKT CD160, was associated with convalescence in moderate patients. In severe patients, the immune landscape featured a deranged interferon response, profound immune exhaustion with skewed T cell receptor repertoire and broad T cell expansion. These findings illustrate the dynamic nature of immune responses during disease progression.
| Type: | Article |
|---|---|
| Title: | Single-cell landscape of immunological responses in patients with COVID-19 |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41590-020-0762-x |
| Publisher version: | https://doi.org/10.1038/s41590-020-0762-x |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. |
| Keywords: | Antimicrobial responses, Viral infection |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10114039 |
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