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The simutaneous immunocytochemical study of CD1A and S100 antigens in cervix and skin: The development and use of an immunocytochemical technique

Maddox, Peter Henry; (1992) The simutaneous immunocytochemical study of CD1A and S100 antigens in cervix and skin: The development and use of an immunocytochemical technique. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

A technique using formalin-calcium pre-fixed frozen sections has been developed which enabled the simultaneous demonstration of CDla and S100 antigens using the Dako antibodies, monoclonal Nal/34 and polyclonal S100 and an avidin-biotin complex peroxidase label. A reproducible counting method has been described which made a direct comparison of these two antigens in the epithelium of human skin and cervix. The Wilcoxon non-parametric test indicated no significant difference between the CDla and S100 counts of normal cervix (Z=1.02) but a difference between the seven counts showing minimal change papilloma virus infection (Z=2.63). A significant difference was shown in biopsies of normal skin (Z=3.37). The same test performed upon biopsies of cervix and skin of patients infected with Human Immune Deficiency Virus gave Z-values of 5.14 and 4.17 respectively. There was no significant difference between the CDla counts of these biopsies and those of normal biopsies (cervix, Z=0.01; skin, Z=0.62), but a significant difference when the same comparison was made for the SlOO counts (cervix, Z=5.17; skin, Z=2.89). The ratio of CD1a/S100 positivity in normal epithelium was found to approach unity, rising in value in cases of minimal change Human Papilloma Virus infection and rising still higher in cases of Human Immune Deficiency Virus infection. The effect of subcutaneous malignant breast disease upon the expression of the two antigens in the overlying epithelia indicated a lower CDla positive count in epithelia distant from the tumour and a normal count above the tumour (Z=2.86). There was no significant difference in the S100 counts of the two sites (Z=0.26). The final study used morphometric and colorimetric methods to support an observation previously reported in cytological material that the two antigens are expressed upon an individual Langerhans cell.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: The simutaneous immunocytochemical study of CD1A and S100 antigens in cervix and skin: The development and use of an immunocytochemical technique
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: Health and environmental sciences; Antigens
URI: https://discovery.ucl.ac.uk/id/eprint/10113808
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