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Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis

Haas, TM; Qiu, D; Häner, M; Angebauer, L; Ripp, A; Singh, J; Koch, H-G; ... Jessen, HJ; + view all (2020) Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis. The Journal of Organic Chemistry 10.1021/acs.joc.0c00841. (In press). Green open access

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Abstract

The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.

Type: Article
Title: Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acs.joc.0c00841
Publisher version: https://doi.org/10.1021/acs.joc.0c00841
Language: English
Additional information: © 2020 American Chemical Society. This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html).
Keywords: Nucleic acids, Peptides and proteins, Monomers,Ketones, Cations
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10113618
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