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Structural predictions of the functions of membrane proteins from HDX-MS

Lau, AM; Jia, R; Bradshaw, RT; Politis, A; (2020) Structural predictions of the functions of membrane proteins from HDX-MS. Biochemical Society Transactions , 48 (3) pp. 971-979. 10.1042/BST20190880. Green open access

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Abstract

HDX-MS has emerged as a powerful tool to interrogate the structure and dynamics of proteins and their complexes. Recent advances in the methodology and instrumentation have enabled the application of HDX-MS to membrane proteins. Such targets are challenging to investigate with conventional strategies. Developing new tools are therefore pertinent for improving our fundamental knowledge of how membrane proteins function in the cell. Importantly, investigating this central class of biomolecules within their native lipid environment remains a challenge but also a key goal ahead. In this short review, we outline recent progresses in dissecting the conformational mechanisms of membrane proteins using HDX-MS. We further describe how the use of computational strategies can aid the interpretation of experimental data and enable visualisation of otherwise intractable membrane protein states. This unique integration of experiments with computations holds significant potential for future applications.

Type: Article
Title: Structural predictions of the functions of membrane proteins from HDX-MS
Open access status: An open access version is available from UCL Discovery
DOI: 10.1042/BST20190880
Publisher version: https://doi.org/10.1042/BST20190880
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, MASS-SPECTROMETRY, CONFORMATIONAL DYNAMICS, HYDROGEN-EXCHANGE, NATIVE-STATE, ION-CHANNEL, MECHANISM, ASSEMBLIES, OLIGOMERIZATION, STOICHIOMETRY, FLUCTUATIONS
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science
URI: https://discovery.ucl.ac.uk/id/eprint/10113208
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