Griffin, John; (1992) The measurement of octadeca-9cis, 11trans-dienoic acid in human neoplasia: Methods and applications. Doctoral thesis (Ph.D), UCL (University College London).

Text The measurement of octadeca-9cis, 11trans-dienoic acid in human neoplasi, Methods and applications.pdf Download (7MB)

Abstract

Diene conjugation is an established marker of free radical attack on lipids. The major diene conjugated compound in human body fluids is octadeca-9cis, 11trans-dienoic acid, 18:2(9c,11t). The serum concentration of 18:2(9c,11t) is significantly raised in some pathological conditions. The method for assaying diene conjugated and non-diene conjugated fatty acids in serum was improved and modified to enable tissue, small biopsies and cells suspensions to be analysed. The assay was automated so that up to 75 specimens could be analysed daily and a computer program written to perform the post-run calculations. The concept of the molar ratio 18:2(9c,11t)/18:2(9c,12c), and its usefulness in a clinical context, was further developed. The molar ratio, 18:2(9c,11t)/18;2(9c,12c), in neoplastic tissue from the cervix uteri (but not colon) was significantly higher than corresponding normal tissue. However, the assay was not sufficiently discriminating for the diagnosis of cervical cancer. A major reason for this was the enzymatic generation of 18:2(9c,11t) in the vagina by commensal bacteria. . It was concluded that changes in the concentrations of 18:2(9c,11t) in man should not be regarded as reflecting free-radical activity alone. By inference total diene conjugation in humans cannot be regarded as a simple function of free-radical damage to lipids.

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