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Combinatorial multivalent interactions drive cooperative assembly of the COPII coat

Stancheva, VG; Li, X-H; Hutchings, J; Gomez-Navarro, N; Santhanam, B; Babu, MM; Zanetti, G; (2020) Combinatorial multivalent interactions drive cooperative assembly of the COPII coat. Journal of Cell Biology , 219 (11) 10.1083/jcb.202007135. Green open access

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Abstract

Protein secretion is initiated at the endoplasmic reticulum by the COPII coat, which self-assembles to form vesicles. Here, we examine the mechanisms by which a cargo-bound inner coat layer recruits and is organized by an outer scaffolding layer to drive local assembly of a stable structure rigid enough to enforce membrane curvature. An intrinsically disordered region in the outer coat protein, Sec31, drives binding with an inner coat layer via multiple distinct interfaces, including a newly defined charge-based interaction. These interfaces combinatorially reinforce each other, suggesting coat oligomerization is driven by the cumulative effects of multivalent interactions. The Sec31 disordered region could be replaced by evolutionarily distant sequences, suggesting plasticity in the binding interfaces. Such a multimodal assembly platform provides an explanation for how cells build a powerful yet transient scaffold to direct vesicle traffic.

Type: Article
Title: Combinatorial multivalent interactions drive cooperative assembly of the COPII coat
Open access status: An open access version is available from UCL Discovery
DOI: 10.1083/jcb.202007135
Publisher version: http://dx.doi.org/10.1083/jcb.202007135
Language: English
Additional information: © 2020 MRC Laboratory of Molecular Biology. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https:// creativecommons.org/licenses/by/4.0/)
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10111463
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