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The human Y chromosome; Towards a physical map in terms of overlapping cosmid clones

Taylor, Kay Elizabeth; (1992) The human Y chromosome; Towards a physical map in terms of overlapping cosmid clones. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

This thesis describes the construction and analysis of a cosmid library specific for the human Y chromosome. The purpose of preparing such a library is to provide a source of ordered overlapping clones for use in the identification of genes, analysis of chromosome structure, and perhaps, as a starting point for the sequencing of the chromosome. Approximately 2,000 cosmid clones containing inserts derived from human DNA have been identified in a library prepared from a somatic cell hybrid containing the Y chromosome as the only human material. These clones have been analysed in two principal ways. Firstly, hybridisation to various characterised DNA fragments has identified cosmids containing known repeat elements and single copy DNA sequences which have been previously assigned to regions of the chromosome by deletion mapping. Secondly, a unique 'fingerprint' has been produced for each clone; band patterns of Hint I digestion products separated by polyacrylamiae gel electrophoresis have been analysed by computer to detect overlapping clones. A significant proportion of the chromosome is represented in this way.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: The human Y chromosome; Towards a physical map in terms of overlapping cosmid clones
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: Biological sciences; Cosmid library
URI: https://discovery.ucl.ac.uk/id/eprint/10111299
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