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Physical mapping of a chromosomal region deleted in small cell lung cancer

Gulati, Kiran; (1992) Physical mapping of a chromosomal region deleted in small cell lung cancer. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

Characteristic deletions have been associated with a number of solid tumours, which represent the sites of tumour suppressor genes. The deletion of 3p21 sequences in small cell lung carcinoma has been demonstrated. To identify and isolate this tumour suppressor gene by positional cloning, a precise knowledge of the boundaries of the deletion is required. A detailed physical map of this region is important in the identification of genes with potential tumour suppressing properties. The thesis describes the physical mapping of a region of the human genome, 3p21, which is consistently deleted in small cell lung carcinoma, SCLC. These deletions are thought to indicate the presence of a tumour suppressor gene. Somatic cell hybrids and pulsed field gel electrophoresis were used to map within the deleted region. Somatic cell hybrids were established to provide a mapping resource for the fine mapping of markers on 3p21. Each panel of hybrids were characterised with available 3p markers and genes. One hybrid containing a portion of 3p21 representing a part of the deletion was used further in physical mapping studies. A gene was isolated from this hybrid and it's genomic structure determined. A cosmid encompassing this gene was used to initiate a chromosomal walk and construction of a contig in this region. Using pulsed field gel electrophoresis, a long range map around this gene was generated and a physical link to another marker in 3p21 shown. This defined region was analysed in SCLC tumours using similar techniques.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: Physical mapping of a chromosomal region deleted in small cell lung cancer
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: Biological sciences; Chromosome mapping
URI: https://discovery.ucl.ac.uk/id/eprint/10111242
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