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Zinc bioglasses regulate mineralization in human dental pulp stem cells

Huang, M; Hill, RG; Rawlinson, SCF; (2017) Zinc bioglasses regulate mineralization in human dental pulp stem cells. Dental Materials , 33 (5) pp. 543-552. 10.1016/j.dental.2017.03.011. Green open access

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Abstract

Objective: A promising strategy in regenerative endodontics is the combination of human dental pulp stem cells (hDPSCs) with an appropriate biomaterial substrate. The effects of zinc and zinc containing bioactive glasses (ZnBGs) on hDPSCs have been characterized in this study. / Methods: ZnBGs were designed and produced. Then the odontogenic differentiation and mineralization potential of hDPSCs upon ZnBGs treatment were investigated. / Results: Free Zn ions (0–5 ppm) enhanced proliferation and alkaline phosphatase (ALP) activity of hDPSCs. Further, ZnBGs conditioned medium (ZnBG-CM) increased the production and secretion of odontogenic markers: dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1). In addition, we identified that mRNA expression of the osteogenic markers RUNX2, OCN, BSP, BMP-2, MEPE and ON was increased following treatment with ZnBG-CM. Long term treatment with ZnBG-CM increases the formation rate of mineralized nodules (similar to hydroxyapatite, Ca:P = 1.6), as confirmed by scanning electron microscopy combined with energy dispersive X-ray spectroscopy (SEM-EDX). Lastly, the administration of ZnBG-CM induces VEGF expression. / Significance: These findings implicate that ZnBG would be beneficial in regenerative endodontics and could influence the way present Zn containing clinical products are used.

Type: Article
Title: Zinc bioglasses regulate mineralization in human dental pulp stem cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.dental.2017.03.011
Publisher version: https://doi.org/10.1016/j.dental.2017.03.011
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Zinc, Bioglass, Dental pulp stem cells, Mineralization, DSPPDMP-1VEGF
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
URI: https://discovery.ucl.ac.uk/id/eprint/10110664
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