UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Direct Transactivation of the Anti-apoptotic Gene Apolipoprotein J (Clusterin) by B-MYB

Cervellera, M; Raschella, G; Santilli, G; Tanno, B; Ventura, A; Mancini, C; Sevignani, C; ... Sala, A; + view all (2000) Direct Transactivation of the Anti-apoptotic Gene Apolipoprotein J (Clusterin) by B-MYB. Journal of Biological Chemistry , 275 (28) pp. 21055-21060. 10.1074/jbc.M002055200. Green open access

[thumbnail of J. Biol. Chem.-2000-Cervellera-21055-60.pdf]
Preview
Text
J. Biol. Chem.-2000-Cervellera-21055-60.pdf - Published version

Download (312kB) | Preview

Abstract

B-MYB is a ubiquitously expressed transcription factor involved in the regulation of cell survival, proliferation, and differentiation. In an attempt to isolate B-MYB-regulated genes that may explain the role of B-MYB in cellular processes, representational difference analysis was performed in neuroblastoma cell lines with different levels of B-MYB expression. One of the genes, the mRNA levels of which were enhanced in B-MYB expressing cells, was ApoJ/Clusterin(SGP-2/TRMP-2) (ApoJ/Clusterin), previously implicated in regulation of apoptosis and tumor progression. Here we show that the human ApoJ/Clusterin gene contains a Myb binding site in its 5\' flanking region, which interacts with bacterially synthesized B-MYB protein and mediates B-MYB-dependent transactivation of the ApoJ/Clusterin promoter in transient transfection assays. Endogenous ApoJ/Clusterin expression is induced in mammalian cell lines following transient transfection of a B-MYB cDNA. Blockage of secreted clusterin by a monoclonal antibody results in increased apoptosis of neuroblastoma cells exposed to the chemotherapeutic drug doxorubicin. Thus, activation of ApoJ/Clusterin by B-MYB may be an important step in the regulation of apoptosis in normal and diseased cells

Type: Article
Title: Direct Transactivation of the Anti-apoptotic Gene Apolipoprotein J (Clusterin) by B-MYB
Open access status: An open access version is available from UCL Discovery
DOI: 10.1074/jbc.M002055200
Publisher version: https://doi.org/10.1074/jbc.M002055200
Language: English
Additional information: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: BC, GENE, Animals Base Sequence COS Cells *Cell Cycle Proteins Clusterin DNA-Binding Proteins/*metabolism Glycoproteins/biosynthesis/*genetics Humans *Molecular Chaperones Molecular Sequence Data Neoplasm Proteins/genetics Neuroblastoma Oncogene Proteins/metabolism
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10110439
Downloads since deposit
8Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item