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Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Huggett, JF; Benes, V; Bustin, SA; Garson, JA; Harris, K; Kammel, M; Kubista, M; ... Zeichhardt, H; + view all (2020) Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2. Clinical Chemistry 10.1093/clinchem/hvaa214. (In press). Green open access

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Abstract

Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.

Type: Article
Title: Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1093/clinchem/hvaa214
Publisher version: https://doi.org/10.1093/clinchem/hvaa214
Language: English
Additional information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: SARS-CoV-2, RT-qPCR, Contamination, False positive, Molecular Diagnosis
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/10109945
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