Huggett, JF;
Benes, V;
Bustin, SA;
Garson, JA;
Harris, K;
Kammel, M;
Kubista, M;
... Zeichhardt, H; + view all
(2020)
Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2.
Clinical Chemistry
10.1093/clinchem/hvaa214.
(In press).
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Abstract
Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.
| Type: | Article |
|---|---|
| Title: | Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2 |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1093/clinchem/hvaa214 |
| Publisher version: | https://doi.org/10.1093/clinchem/hvaa214 |
| Language: | English |
| Additional information: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | SARS-CoV-2, RT-qPCR, Contamination, False positive, Molecular Diagnosis |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10109945 |
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