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Ethylene glycol and glycolic acid production by wild-type Escherichia coli

Lu, X; Yao, Y; Yang, Y; Zhang, Z; Gu, J; Mojovic, L; Knezevic-Jugovic, Z; ... Hao, J; + view all (2020) Ethylene glycol and glycolic acid production by wild-type Escherichia coli. Biotechnology and Applied Biochemistry , 68 (4) pp. 744-755. 10.1002/bab.1987. Green open access

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Abstract

Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild‐type Escherichia coli W3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore, E. coli strains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced by E. coli ΔyqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, the E. coli ΔaldA+yqhD produced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production by E. coli on xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.

Type: Article
Title: Ethylene glycol and glycolic acid production by wild-type Escherichia coli
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/bab.1987
Publisher version: https://doi.org/10.1002/bab.1987
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: ethylene glycol; glycolic acid; xylonic acid; xylose; E. coli
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10109792
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