Carugo, D;
Owen, J;
Crake, C;
Lee, JY;
Stride, E;
(2015)
Biologically and Acoustically Compatible Chamber for Studying Ultrasound-Mediated Delivery of Therapeutic Compounds.
Ultrasound in Medicine & Biology
, 41
(7)
pp. 1927-1937.
10.1016/j.ultrasmedbio.2015.03.020.
Preview |
Text
PIIS0301562915002331.pdf - Published Version Download (2MB) | Preview |
Abstract
Ultrasound (US), in combination with microbubbles, has been found to be a potential alternative to viral therapies for transfecting biological cells. The translation of this technique to the clinical environment, however, requires robust and systematic optimization of the acoustic parameters needed to achieve a desired therapeutic effect. Currently, a variety of different devices have been developed to transfect cells in vitro, resulting in a lack of standardized experimental conditions and difficulty in comparing results from different laboratories. To overcome this limitation, we propose an easy-to-fabricate and cost-effective device for application in US-mediated delivery of therapeutic compounds. It comprises a commercially available cell culture dish coupled with a silicon-based “lid” developed in-house that enables the device to be immersed in a water bath for US exposure. Described here are the design of the device, characterization of the sound field and fluid dynamics inside the chamber and an example protocol for a therapeutic delivery experiment.
| Type: | Article |
|---|---|
| Title: | Biologically and Acoustically Compatible Chamber for Studying Ultrasound-Mediated Delivery of Therapeutic Compounds |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ultrasmedbio.2015.03.020 |
| Publisher version: | https://doi.org/10.1016/j.ultrasmedbio.2015.03.020 |
| Language: | English |
| Additional information: | © 2015 The Authors. Published by Elsevier Inc. on behalf of World Federation for Ultrasound in Medicine & Biology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Ultrasound-mediated drug delivery, Small interfering RNA, In vitro assay, Cell culture device, Sonoporation |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10109415 |
Archive Staff Only
 |
View Item |
