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Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles

Carugo, D; Aron, M; Sezgin, E; de la Serna, JB; Kuirnova, MK; Eggeling, C; Stride, E; (2017) Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles. Biomaterials , 113 pp. 105-117. 10.1016/j.biomaterials.2016.10.034. Green open access

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Abstract

The transfer of material from phospholipid-coated microbubbles to cell membranes has been hypothesized to play a role in ultrasound-mediated drug delivery. In this study, we employed quantitative fluorescence microscopy techniques to investigate this phenomenon in both artificial and biological membrane bilayers in an acoustofluidic system. The results of the present study provide strong evidence for the transfer of material from microbubble coatings into cell membranes. Our results indicate that transfer of phospholipids alters the organization of molecules in cell membranes, specifically the lipid ordering or packing, which is known to be a key determinant of membrane mechanical properties, protein dynamics, and permeability. We further show that polyethylene-glycol, used in many clinical microbubble formulations, also has a major impact on both membrane lipid ordering and the extent of lipid transfer, and that this occurs even in the absence of ultrasound exposure

Type: Article
Title: Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.biomaterials.2016.10.034
Publisher version: https://doi.org/10.1016/j.biomaterials.2016.10.034
Language: English
Additional information: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Microbubbles, Ultrasound, Lipid order, Membrane bilayers
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: https://discovery.ucl.ac.uk/id/eprint/10109406
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