Owen, J;
Crake, C;
Lee, JY;
Carugo, D;
Beguin, E;
Khrapitchev, AA;
Browning, RJ;
... Stride, E; + view all
(2018)
A versatile method for the preparation of particle-loaded microbubbles for multimodality imaging and targeted drug delivery.
Drug Delivery and Translational Research
, 8
pp. 342-356.
10.1007/s13346-017-0366-7.
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Abstract
Microbubbles are currently in clinical use as ultrasound contrast agents and under active investigation as mediators of ultrasound therapy. To improve the theranostic potential of microbubbles, nanoparticles can be attached to the bubble shell for imaging, targeting and/or enhancement of acoustic response. Existing methods for fabricating particle-loaded bubbles, however, require the use of polymers, oil layers or chemical reactions for particle incorporation; embed/attach the particles that can reduce echogenicity; impair biocompatibility; and/or involve multiple processing steps. Here, we describe a simple method to embed nanoparticles in a phospholipid-coated microbubble formulation that overcomes these limitations. Magnetic nanoparticles are used to demonstrate the method with a range of different microbubble formulations. The size distribution and yield of microbubbles are shown to be unaffected by the addition of the particles. We further show that the microbubbles can be retained against flow using a permanent magnet, can be visualised by both ultrasound and magnetic resonance imaging (MRI) and can be used to transfect SH-SY5Y cells with fluorescent small interfering RNA under the application of a magnetic field and ultrasound field.
| Type: | Article |
|---|---|
| Title: | A versatile method for the preparation of particle-loaded microbubbles for multimodality imaging and targeted drug delivery |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s13346-017-0366-7 |
| Publisher version: | https://doi.org/10.1007/s13346-017-0366-7 |
| Language: | English |
| Additional information: | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
| Keywords: | Microbubbles . Ultrasound . Contrast agents . Magnetic . Targeting . Drug delivery |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10109396 |
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