Sezgin, E;
Carugo, D;
Levental, I;
Stride, E;
Eggeling, C;
(2020)
Creating supported plasma membrane bilayers using acoustic pressure.
Membranes
, 10
(2)
, Article 30. 10.3390/membranes10020030.
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Abstract
Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics.
| Type: | Article |
|---|---|
| Title: | Creating supported plasma membrane bilayers using acoustic pressure |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3390/membranes10020030 |
| Publisher version: | https://doi.org/10.3390/membranes10020030 |
| Language: | English |
| Additional information: | © 2020 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | GPMVs; acoustic pressure; supported bilayers; plasma membrane vesicles; plasma membrane bilayers |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10109385 |
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