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Flow, suspension, and mixing dynamics in DASGIP bioreactors: Part 1

Samaras, JJ; Micheletti, M; Ducci, A; (2020) Flow, suspension, and mixing dynamics in DASGIP bioreactors: Part 1. AIChE Journal 10.1002/aic.17014. (In press). Green open access

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Abstract

The bioreactor flow environment has a significant impact on process performance, especially in stem cell cultures. The work of Correia et al found intermittent agitation modes to improve induced pluripotent stem cell (iPSC)‐cardiomyocyte differentiation yields; however, to date, the impact within the flow has not been fully characterized. This work aims to characterize the flow dynamics occurring within a commercially available DASGIP bioreactor, equipped with a two‐blade paddle impeller, operating under different agitation modes and for two bottom geometries. The paddle impeller configuration generated an axial flow profile due to a large impeller D/T and blade confinement with the bioreactor wall. The application of intermittent agitation was shown to induce two transient spikes in flow velocity and shear stress, the amplification of which increased with dwell duration. Marginally increasing the dwell duration was shown previously to increase differentiation yields, therefore it can be stipulated that introduction of these spikes was favorable toward cardiogenic differentiation.

Type: Article
Title: Flow, suspension, and mixing dynamics in DASGIP bioreactors: Part 1
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/aic.17014
Publisher version: https://doi.org/10.1002/aic.17014
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10108556
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