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Dynamics of ligand binding to a rigid glycosidase

Ubbink, M; Ben Bdira, F; Waudby, CA; Volkov, AN; Schröder, SP; Ab, E; Codée, JDC; ... van Ingen, H; + view all (2020) Dynamics of ligand binding to a rigid glycosidase. Angewandte Chemie International Edition 10.1002/anie.202003236. (In press). Green open access

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Abstract

The single-domain GH11 glycosidase from  Bacillus circulans  (BCX) is involved in the degradation of hemicellulose, one of the most abundant renewable biomaterials in nature. We demonstrate that BCX in solution undergoes minimal structural changes during turnover. NMR spectroscopy results show that the rigid protein matrix provides a frame for fast substrate binding in multiple conformations, accompanied by slow conversion, attributed to an enzyme induced substrate distortion. A model is proposed in which the rigid enzyme takes advantage of substrate flexibility to induce a conformation that facilitates the acyl formation step of the hydrolysis reaction.

Type: Article
Title: Dynamics of ligand binding to a rigid glycosidase
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/anie.202003236
Publisher version: http://dx.doi.org/10.1002/anie.202003236
Language: English
Additional information: This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial, and no modifications or adaptations are made. See here for more information: https://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords: Glycosidases, NMR spectroscopy, dynamics, ligand binding, rigid fold
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10107681
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