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A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity

Yang, T; Cheong, A; Mai, X; Zou, S; Woon, ECY; (2016) A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity. Chemical Communications , 52 (36) pp. 6181-6184. 10.1039/c6cc01045h. Green open access

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Abstract

We describe a novel methylation-sensitive nucleic acid (RNA) probe which switches conformation according to its methylation status. When combined with a differential scanning fluorimetry technique, it enables highly sensitive and selective detection of demethylase activity at a single methylated-base level. The approach is highly versatile and may be adapted to a broad range of RNA demethylases.

Type: Article
Title: A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/c6cc01045h
Publisher version: https://doi.org/10.1039/C6CC01045H
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10107019
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