Yang, T;
Cheong, A;
Mai, X;
Zou, S;
Woon, ECY;
(2016)
A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity.
Chemical Communications
, 52
(36)
pp. 6181-6184.
10.1039/c6cc01045h.
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Abstract
We describe a novel methylation-sensitive nucleic acid (RNA) probe which switches conformation according to its methylation status. When combined with a differential scanning fluorimetry technique, it enables highly sensitive and selective detection of demethylase activity at a single methylated-base level. The approach is highly versatile and may be adapted to a broad range of RNA demethylases.
| Type: | Article |
|---|---|
| Title: | A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/c6cc01045h |
| Publisher version: | https://doi.org/10.1039/C6CC01045H |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10107019 |
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