UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Early Polylysine Release from Dental Composites and Its Effects on Planktonic Streptococcus mutans Growth

Lygidakis, NN; Allan, E; Xia, W; Ashley, PF; Young, AM; (2020) Early Polylysine Release from Dental Composites and Its Effects on Planktonic Streptococcus mutans Growth. Journal of Functional Biomaterials , 11 (3) , Article 53. 10.3390/jfb11030053. Green open access

[thumbnail of jfb-11-00053.pdf]
Preview
Text
jfb-11-00053.pdf - Published Version

Download (2MB) | Preview

Abstract

The study aim was to assess the effect of incorporating polylysine (PLS) filler at different mass fractions (0.5, 1 and 2 wt%) on PLS release and Streptococcus mutans planktonic growth. Composite containing PLS mass and volume change and PLS release upon water immersion were assessed gravimetrically and via high-performance liquid chromatography (HPLC), respectively. Disc effects on bacterial counts in broth initially containing 8 × 10^{5} versus 8 × 10^{6} CFU/mL Streptococcus mutans UA159 were determined after 24 h. Survival of sedimented bacteria after 72 h was determined following LIVE/DEAD staining of composite surfaces using confocal microscopy. Water sorption-induced mass change at two months increased from 0.7 to 1.7% with increasing PLS concentration. Average volume increases were 2.3% at two months whilst polylysine release levelled at 4% at 3 weeks irrespective of composite PLS level. Early percentage PLS release, however, was faster with higher composite content. With 0.5, 1 and 2% polylysine initially in the composite filler phase, 24-h PLS release into 1 mL of water yielded 8, 25 and 93 ppm respectively. With initial bacterial counts of 8 × 10^{5} CFU/mL, this PLS release reduced 24-h bacterial counts from 10^{9} down to 10^{8}, 10^{7} and 10^{2} CFU/mL respectively. With a high initial inoculum, 24-h bacterial counts were 10^{9} with 0, 0.5 or 1% PLS and 10^{7} with 2% PLS. As the PLS composite content was raised, the ratio of dead to live sedimented bacteria increased. The antibacterial action of the experimental composites could reduce residual bacteria remaining following minimally invasive tooth restorations.

Type: Article
Title: Early Polylysine Release from Dental Composites and Its Effects on Planktonic Streptococcus mutans Growth
Location: Switzerland
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/jfb11030053
Publisher version: https://doi.org/10.3390/jfb11030053
Language: English
Additional information: © 2020 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Keywords: antibacterial, dental composites, polylysine
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > EDI Craniofacial and Development Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10106712
Downloads since deposit
41Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item