Wyrobnik, TA;
Ducci, A;
Micheletti, M;
(2020)
Advances in human mesenchymal stromal cell-based therapies – Towards an integrated biological and engineering approach.
Stem Cell Research
, 47
, Article 101888. 10.1016/j.scr.2020.101888.
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Abstract
Recent advances of stem cell-based therapies in clinical trials have raised the need for large-scale manufacturing platforms that can supply clinically relevant doses to meet an increasing demand. Promising results have been reported using stirred-tank bioreactors, where human Mesenchymal Stromal Cells (hMSCs) were cultured in suspension on microcarriers (MCs), although the formation of microcarrier-cell-aggregates might still limit mass transfer and determine a heterogeneous distribution of hMSCs. A variety of MCs, bioreactor-impeller configurations, and agitation conditions have been established in an attempt to overcome the trade-off of ensuring good suspension while keeping the stresses to a minimum. While understanding and controlling the fluid flow environment of bioreactors has been initially under-appreciated, it has recently gained in popularity in the mission of providing ideal culture environments across different scales. This review article aims to provide a comprehensive overview of how rigorous engineering characterisation studies improved the outcome of biological process development and scale-up efforts. Reconciling these two disciplines is crucial to propose tailored bioprocessing solutions that can provide improved growth environments across a range of scales for the allogeneic cell therapies of the future.
| Type: | Article |
|---|---|
| Title: | Advances in human mesenchymal stromal cell-based therapies – Towards an integrated biological and engineering approach |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.scr.2020.101888 |
| Publisher version: | https://doi.org/10.1016/j.scr.2020.101888 |
| Language: | English |
| Additional information: | © 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
| Keywords: | Mesenchymal stromal cell bioprocessing, Impeller design, Stirred-tank bioreactors, Cell therapy process development |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10106460 |
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