Griffiths, VA;
Valera, AM;
Lau, JYN;
Ros, H;
Younts, TJ;
Marin, B;
Baragli, C;
... Silver, RA; + view all
(2020)
Real-time 3D movement correction for two-photon imaging in behaving animals.
Nature Methods
, 17
pp. 741-748.
10.1038/s41592-020-0851-7.
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Abstract
Two-photon microscopy is widely used to investigate brain function across multiple spatial scales. However, measurements of neural activity are compromised by brain movement in behaving animals. Brain motion-induced artifacts are typically corrected using post hoc processing of two-dimensional images, but this approach is slow and does not correct for axial movements. Moreover, the deleterious effects of brain movement on high-speed imaging of small regions of interest and photostimulation cannot be corrected post hoc. To address this problem, we combined random-access three-dimensional (3D) laser scanning using an acousto-optic lens and rapid closed-loop field programmable gate array processing to track 3D brain movement and correct motion artifacts in real time at up to 1 kHz. Our recordings from synapses, dendrites and large neuronal populations in behaving mice and zebrafish demonstrate real-time movement-corrected 3D two-photon imaging with submicrometer precision.
| Type: | Article |
|---|---|
| Title: | Real-time 3D movement correction for two-photon imaging in behaving animals |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41592-020-0851-7 |
| Publisher version: | https://doi.org/10.1038/s41592-020-0851-7 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Biochemistry & Molecular Biology, COMPACT ACOUSTOOPTIC LENS, CELLULAR-RESOLUTION, NEURAL ACTIVITY, MOTION ARTIFACTS, BRAIN, AWAKE, MICROSCOPY, SCALE |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10106242 |
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