Stones, Robert William;
(1994)
Local vascular control in the human ovarian circulation.
Doctoral thesis (M.D), UCL (University College London).
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Abstract
OBJECTIVE: The aims of these studies were i) to undertake a descriptive study of the ultrastructural characteristics, innervation and pharmacological responsiveness of the human ovarian vein and ii) to develop an in vitro isolated perfused premenopausal human ovary model in order to investigate the role of adenosine 5'-triphosphate and regulatory peptides in local vascular control in the ovary. DESIGN: The pattern of innervation was investigated using image analysis of electron micrographs, histofluorescence and immunohistochemistry of cryostat sections and whole mount stretch preparations of ovarian vein. In situ electron microscopic immunolocalization of Substance P and immunolocalization of Substance P and other vasoactive agents in cultured ovarian venous endothelium were performed. Pharmacological responses to electrical stimulation of sympathetic nerves and various agents were studied and endothelium-mediated relaxation responses were investigated. The ovarian vascular bed was perfused in vitro with a physiological solution and adenosine 5'-triphosphate and peptide release was estimated in perfusate. RESULTS: Ultrastructural features of the ovarian vein were established. Substance P, 5- hydroxytryptamine. Neuropeptide Y and Endothelin were localized in endothelial cells. Innervation was predominantly sympathetic. In organ bath studies evidence for purinergic and adrenergic cotransmission in sympathetic nerves was obtained. A loss of sensitivity to 5-hydroxytryptamine was noted in specimens from women who had previously undergone sterilization. Relaxation to Substance P was partially mediated via nitric oxide. Release of adenosine 5'-triphosphate and peptides from the perfused ovary was demonstrated and responses seen to purine analogues and peptides. CONCLUSION: Local vascular control in the human ovarian circulation includes both endothelial and sympathetic nerve-mediated mechanisms.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | M.D |
| Title: | Local vascular control in the human ovarian circulation |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Thesis digitised by ProQuest. |
| Keywords: | Biological sciences; Ovarian veins |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10105975 |
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