UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization

Cao, L; Yonis, A; Vaghela, M; Barriga, EH; Chugh, P; Smith, MB; Maufront, J; ... Charras, G; + view all (2020) SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization. Nature Cell Biology , 22 pp. 803-814. 10.1038/s41556-020-0531-y. Green open access

[thumbnail of Accepted Manuscript]
Preview
Text (Accepted Manuscript)
Charras_SPIN90 associates with mDia1 and the Arp2:3 complex to regulate cortical actin organization_AAM.pdf - Accepted Version

Download (3MB) | Preview
[thumbnail of Supplementary Material]
Preview
Text (Supplementary Material)
Charras_SPIN90 associates with mDia1 and the Arp2:3 complex to regulate cortical actin organization_SuppM.pdf - Accepted Version

Download (3MB) | Preview

Abstract

Cell shape is controlled by the submembranous cortex, an actomyosin network mainly generated by two actin nucleators: the Arp2/3 complex and the formin mDia1. Changes in relative nucleator activity may alter cortical organization, mechanics and cell shape. Here we investigate how nucleation-promoting factors mediate interactions between nucleators. In vitro, the nucleation-promoting factor SPIN90 promotes formation of unbranched filaments by Arp2/3, a process thought to provide the initial filament for generation of dendritic networks. Paradoxically, in cells, SPIN90 appears to favour a formin-dominated cortex. Our in vitro experiments reveal that this feature stems mainly from two mechanisms: efficient recruitment of mDia1 to SPIN90-Arp2/3 nucleated filaments and formation of a ternary SPIN90-Arp2/3-mDia1 complex that greatly enhances filament nucleation. Both mechanisms yield rapidly elongating filaments with mDia1 at their barbed ends and SPIN90-Arp2/3 at their pointed ends. Thus, in networks, SPIN90 lowers branching densities and increases the proportion of long filaments elongated by mDia1.

Type: Article
Title: SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41556-020-0531-y
Publisher version: https://doi.org/10.1038/s41556-020-0531-y
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/10104055
Downloads since deposit
255Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item