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Cellular and molecular studies related to a cell line of the oligodendrocyte-type-2 astrocyte lineage derived from a human glioblastoma multiforme

Gutowski, Nicholas Jan; (1995) Cellular and molecular studies related to a cell line of the oligodendrocyte-type-2 astrocyte lineage derived from a human glioblastoma multiforme. Doctoral thesis (M.D), UCL (University College London). Green open access

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Abstract

By using techniques from studies of the rodent oligodendrocyte-type-2 astrocyte (0-2A) lineage, the Human 0-2A lineage Glioblastoma number 1 (Hu-O-2A/Gbl) cell line was grown from a human glioblastoma multiforme. This thesis describes findings related to this cell line. Receptor tyrosine kinases and other tyrosine kinase complementary DNA fragments were cloned from the cell line to provide information about growth factors to which the cells might respond. Consequently, it was found that the cells showed mitogenic and antigenic changes in several culture conditions, similar to that seen for rodent 0-2A progenitor cells. The cloning data also suggested that Vascular Endothelial Growth Factor (VEGF) might affect these cells. It was found that VEGF enhanced oligodendrocyte maturation in the Hu-0-2A/Gbl cell line and also in rodent oligodendrocytes in vitro. In addition, the HEK2 receptor tyrosine kinase found during initial cloning from the Hu-0-2A/Gbl cell line appears to be a helpful, but not entirely specific, marker for 0-2A lineage cells. A new protein kinase LIMK was cloned from the complementary DNA library of the Hu-0-2A/Gbl cell line. LIMK is predominantly expressed in the nervous system and high expression was found in malignant glioma cell populations, their original tumours and also during human embryonic development.

Type: Thesis (Doctoral)
Qualification: M.D
Title: Cellular and molecular studies related to a cell line of the oligodendrocyte-type-2 astrocyte lineage derived from a human glioblastoma multiforme
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: Biological sciences; Glioblastoma multiforme
URI: https://discovery.ucl.ac.uk/id/eprint/10103224
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