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A study of the typing of the genus Enterococcus

Lacoux, Philippe André; (1994) A study of the typing of the genus Enterococcus. Doctoral thesis (M.D.), UCL (University College London). Green open access

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Abstract

Restriction fragment length polymorphism (RFLP) of chromosomal DNA for the intra-species characterisation of enterococci is reported. The bacterial DNA was extracted by a rapid method and digested to provide a characteristic DNA banding pattern after agarose gel electrophoresis. Five different restriction endonucleases Bam HI, Sac I, Sal I, Eco RI and Hind III were assessed for their ability to provide easily readable banding patterns that could be used to differentiate between a representative sample of enterococcal isolates. One hundred and eighty enterococcal isolates from three different sources were then examined using the restriction endonuclease Sal I, and this provided 39 distinct DNA banding patterns; this technique provided a discriminatory method of isolate characterisation for E. faecalis, E. faecium and E. durans. Results were compared with biotyping with the API 20 Strep kit, and with antibiogram data using eight antibiotic impregnated discs. The technique of restriction endonuclease analysis with Sal I was applied to the temporal analysis of faecal enterococci from an isolated Antarctic community, and the results compared with biotyping and antibiogram data. Southern blots were probed with ribosomal RNA from Escherichia coli after digestion of the bacterial DNA with either Eco RI or Hind III. This revealed some potential as a method for differentiation within the enterococcal species, and also at the inter-species level although E. faecium and E. durans were difficult to separate. Recent clinical findings suggest that a typing scheme for S. bovis could be important. These developments are discussed, and the methods above applied to three S. bovis isolates. The historical taxonomic changes within the enterococci are reviewed. The clinical importance of the enterococci is discussed in relation to both the changing pattern of antimicrobial resistance and to the growth in nosocomial infection. Methods of typing the enterococci are reviewed, and the present position of genetic typing methods is discussed.

Type: Thesis (Doctoral)
Qualification: M.D.
Title: A study of the typing of the genus Enterococcus
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
URI: https://discovery.ucl.ac.uk/id/eprint/10102785
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