Saboor, Syed Abdul; (1995) Detection of mycobacterial DNA in tuberculosis and sarcoidosis. Doctoral thesis (M.D), UCL (University College London).

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Abstract

The Polymerase Chain Reaction (PCR) was used to specifically amplify mycobacterial DNA in bronchoalveolar lavage (BAL) samples from patients with tuberculosis (TB), sarcoidosis and other illnesses. Two PCR reactions were employed, the first to specifically detect Mycobacterium tuberculosis complex using the DNA sequences of the M. tuberculosis complex-specific insertion sequence IS986/IS6110. The second PCR employed DNA sequences to conserved sequences of the mycobacterial groEL gene in order to amplify and detect DNA from mycobacteria other than M. tuberculosis. Ninety six clinical samples from ninety six patients were included in the study. The PCR was found to have a greater sensitivity than culture for the diagnosis of tuberculosis; however positive PCR results for M. tuberculosis were found in 9% of patients in which a clinical diagnosis of tuberculosis was never made. M. tuberculosis DNA was detected in 50% of sarcoidosis patients and non-tuberculous mycobacterial DNA detected in a further 20%. Our results demonstrate that a significant proportion of the sarcoidosis patients in this study have mycobacteria in their lungs and most of these mycobacteria belong to M. tuberculosis complex, suggesting a possible etiological role for mycobacteria in sarcoidosis.

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