McKie, Judith Mary; (1998) Molecular characterisation of murine Nfe2l1. Doctoral thesis (Ph.D), UCL (University College London).

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Abstract

The conservation of some developmental processes amongst species as diverse as Drosophila, C.elegans, man and mouse has been one of the most exciting scientific discoveries in recent years. The initial premise for the thesis was the isolation of a mouse cDNA fragment, 8dl, which had striking similarity to a Drosophila protein, CNC. cnc encodes a bZIP transcription factor and is thought to be involved in head specification. The initial aims were therefore to establish if the murine homologue (latterly known as Nfe2l1) of CNC had been isolated, and what, if any, was its role in murine development. The full length sequence of Nfe2l1 was determined and its chromosomal localisation in both mouse and man identified. The expression pattern of Nfe2l1 throughout murine development was studied, and although it was ubiquitous throughout the developmental stages studied, specific sites of developmental upregulation could be identified. Nfe2l1 is unlikely to be the murine homologue of CNC, but rather, one member of a family of CNC-related proteins that form a subclass of the bZIP transcription factor family. The evidence from both the work presented in this thesis and the published studies suggests that Nfe2l1 is likely to have a role in murine development. In addition, a novel human family member, NFM, has been identified by database screening with NFE2L1. Several cDNAs have been isolated and partially characterised by sequence and northern analyses, and NFM-positive human PACs have permitted FISH localisation studies.

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