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Genes encoding rhoptry proteins of the rodent malaria parasite <italic> Plasmodium yoelii</italic> YM

Green, Judith Louise; (1998) Genes encoding rhoptry proteins of the rodent malaria parasite <italic> Plasmodium yoelii</italic> YM. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

The 235 kDa merozoite rhoptry proteins of the rodent malaria parasite Plasmodium yoelii are encoded by a multigene family, members of which are present on several of the parasite's chromosomes. The proteins have been implicated in the specificity of red blood cell invasion observed for different strains of the Plasmodium yoelii parasite. Although partial coding sequence for two of the members of the family, designated E3 and E8, has been previously described, the entire coding sequence for a member of the multigene family has not. In this thesis, the complete coding sequence for the E8 gene is described. The sequence was obtained by the construction, and subsequent screening of, a Plasmodium yoelii YM genomic library. The structure of the gene is similar to that seen for many Plasmodium genes, with an intron separating a short signal sequence from the main body of the coding sequence. The intron is bounded by consensus eukaryotic splice sites at its 5' and 3' ends. The putative signal sequence itself contains all the features associated with a classical signal sequence, with positively charged residues followed by a largely hydrophobic core bounded by charged amino acid residues. In addition to the E8 gene, partial sequence encoding three other members of the multigene family was obtained. Each of these had an extremely high homology to the E8 gene. All of the members of the gene family demonstrated sequence homology to a family of Plasmodium vivax reticulocyte binding proteins—PvRBP-1 and PvRBP-2. The significance of such homology is discussed, as well as further similarity to an integrin homologue protein of Saccharomyces cerevisiae.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: Genes encoding rhoptry proteins of the rodent malaria parasite <italic> Plasmodium yoelii</italic> YM
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: Biological sciences; Plasmodium yoelii
URI: https://discovery.ucl.ac.uk/id/eprint/10100033
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