Griffiths, Brigitte M; (1998) Dentine permeability, adhesive penetration and interfacial stress: A confocal microscope study. Doctoral thesis (Ph.D), UCL (University College London).

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Abstract

Dentine has a heterogeneous structure and is inherently wet due to the presence of fluid filled tubules. Bonding to dentine has not met with the same success as enamel bonding, and dentine bonding systems continue to be developed. In vitro evaluation of these systems centres around interfacial morphology; SEM or TEM (involving considerable sample preparation), microleakage and bond testing. Dynamic interfacial performance with minimal sample preparation has not been evaluated. To address this, techniques were developed to record the appearance of dentine/restorative interfaces in real time, during and after the placement of restorations, in addition to interfacial micropermeability and dynamic performance under load.Teeth, maintained in near physiologic conditions, were restored with a dentine bonding system, in different cavity configurations and interfacial regions examined using fluorescence confocal microscopy (tandem scanning and laser scanning microscopes). Fluorescent dyes were added to the components of the dentine bonding systems and pulpal fluid to clarify the location of the components within the dentine and highlight any micro-permeability. Images were captured on 35mm film and/or video. In addition, a range of computer software programmes were used to capture, edit and store video rate image sequences. Fracture experiments were conducted in shear mode with tooth samples held in a custom made jig with load cell and computer controlled servo-motor pusher to load the sample. Real time images of the interfaces during failure were recorded along with synchronised load data. This allowed the dynamic patterns of interfacial failure to be recorded and categorised for the first time. The fluorescence confocal microscopy techniques provided an in vitro evaluation of dentine permeability, adhesive penetration and interfacial performance under stress, and enabled video rate recording of events at the interface. These techniques have been used in the comparison of performance of dentine bonding systems and have also been influential in the development stage of new materials.

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