Brown, Robert Anthony;
(2002)
Enhanced definition and process confidence during fermentation via direct product monitoring.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
Traditionally it is not common to monitor fermentations closely, especially at the start of a process when biomass and product levels are low. Rapid, direct product monitoring is also uncommon during fermentation due to the time-consuming sample preparation and analysis procedures commonly required. This thesis aims to introduce the concept of rapid, direct data availability, and decision-making arising from this, to the early part of the fermentation process. If data is available in a rapid manner, decisions affecting the outcome of the process may be taken. Thus, conditions deviating from those desirable can be detected and appropriate actions taken. Fermentations have been performed on recombinant Escherichia coli producing a constitutively expressed product in both batch and fed-batch fermentation modes. The results have been used for fault detection, to ascertain as quickly as possible when an abnormal situation arises. Scale-up of fermentation has also been investigated. The fermentation of Saccharomyces cerevisiae in fed-batch culture producing an induced product has also been investigated, with the results gained used to facilitate modelling of the growth and induction phases of the fermentation. Finally, rapid, real-time availability of product titres throughout a complete production process is used to illustrate the ability to describe bioprocesses in terms of product. This can be used for fault detection and estimation of the success of unit operations at all stages of the process. Also, the techniques used enable mass-balancing throughout the process and hence are a tool for increasing process confidence. Possibilities for decision making and control based on the data availability will also exist at certain stages within the process.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | Enhanced definition and process confidence during fermentation via direct product monitoring. |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Thesis digitised by ProQuest. |
| Keywords: | Pure sciences; Applied sciences; Fermentation |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10099101 |
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