Eglin, Roger Charles; (1996) Effect of organic solvents on cell stability. Doctoral thesis (Ph.D), UCL (University College London).

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Abstract

Many existing and novel approaches to bioprocessing using different types of reaction media have evolved in recent years involving biocatalysis of poorly water soluble compounds eg water-miscible cosolvent aqueous, aqueous-organic biphasic, reversed micellar and super critical fluid media. Commercial exploitation of the appropriate media and its engineering characteristics will require detailed knowledge of the media and its engineering characteristics with regards to biocatalyst stability. This thesis examines the effect of membrane integrity as measured by the capacitance from cell beta-dispersion. It relates this to methods of determining cell viability, reactor mixing, and the effect of organic solvents with similar hydrophobic characteristics, as measured by the log of partitioning (log P) between octanol and water, which has become the standard approach. The effect of organic solvents on endogenous uptake rate is also considered. These factors are related to the transfer of organic solvent into the cell. The test organisms selected were: two yeast strains, a bacteria, a fungus and an animal cells line.

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