UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Effect of organic solvents on cell stability

Eglin, Roger Charles; (1996) Effect of organic solvents on cell stability. Doctoral thesis (Ph.D), UCL (University College London). Green open access

[thumbnail of Effect_of_organic_solvents_on_.pdf] Text
Effect_of_organic_solvents_on_.pdf

Download (13MB)

Abstract

Many existing and novel approaches to bioprocessing using different types of reaction media have evolved in recent years involving biocatalysis of poorly water soluble compounds eg water-miscible cosolvent aqueous, aqueous-organic biphasic, reversed micellar and super critical fluid media. Commercial exploitation of the appropriate media and its engineering characteristics will require detailed knowledge of the media and its engineering characteristics with regards to biocatalyst stability. This thesis examines the effect of membrane integrity as measured by the capacitance from cell beta-dispersion. It relates this to methods of determining cell viability, reactor mixing, and the effect of organic solvents with similar hydrophobic characteristics, as measured by the log of partitioning (log P) between octanol and water, which has become the standard approach. The effect of organic solvents on endogenous uptake rate is also considered. These factors are related to the transfer of organic solvent into the cell. The test organisms selected were: two yeast strains, a bacteria, a fungus and an animal cells line.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: Effect of organic solvents on cell stability
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: Applied sciences; Bioprocessing
URI: https://discovery.ucl.ac.uk/id/eprint/10098287
Downloads since deposit
41Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item