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Proton and carbon-13 NMR lipid profiles of cells, tissues and body fluids

Choi, Gregory Tak-Yu; (1994) Proton and carbon-13 NMR lipid profiles of cells, tissues and body fluids. Doctoral thesis (Ph.D.), University College London (United Kingdom). Green open access

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Abstract

This thesis describes the development of a novel, comprehensive and rapid biological lipid analysis via high-field NMR. Based upon the information obtained from various 2-dimensional (2D) NMR experiments, and by comparing NMR spectra of biological lipid extracts to standard lipid spectra, plus cross-references with existing data when available, the major lipids and fatty acids have been successfully identified and quantified. Significantly, information obtained from NMR experiments proved to be in agreement with previous published data, which was obtained using well-established but time-consuming conventional chromatographic techniques. The NMR lipid assay was then applied to the analysis of various biological tissues, cells and body fluids including rat liver, brain, heart, kidney, mast cell, human erythrocyte and plasma lipid extract. Assignments were mostly achieved using 1H- and 13C-NMR spectroscopy, although experiments involved other nuclei were also performed when necessary (e.g. 31P). Applications to the detection of lipid and phospholipid changes in carbon tetrachloride treated animals were carried out.

Type: Thesis (Doctoral)
Qualification: Ph.D.
Title: Proton and carbon-13 NMR lipid profiles of cells, tissues and body fluids
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: (UMI)AAIU063799; Biological sciences; Health and environmental sciences
URI: https://discovery.ucl.ac.uk/id/eprint/10098274
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