UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Kanamycin uptake into Escherichia coli is facilitated by OmpF and OmpC porin channels located in the outer membrane

Bafna, JA; Sans-Serramitjana, E; Acosta-Gutierrez, S; Bodrenko, IV; H Ouml R Ouml Mp Ouml Li, D; Berscheid, A; Broetz-Oesterhelt, H; ... Ceccarelli, M; + view all (2020) Kanamycin uptake into Escherichia coli is facilitated by OmpF and OmpC porin channels located in the outer membrane. ACS Infectious Diseases , 6 (7) pp. 1855-1865. 10.1021/acsinfecdis.0c00102. Green open access

[thumbnail of Acosta Gutierrez_Manuscript_rev_final.pdf]
Preview
Text
Acosta Gutierrez_Manuscript_rev_final.pdf - Accepted Version

Download (1MB) | Preview

Abstract

Despite decades of therapeutic application of aminoglycosides, it is still a matter of debate if porins contribute to the translocation of the antibiotics across the bacterial outer membrane. Here, we quantified the uptake of kanamycin across the major porin channels OmpF and OmpC present in the outer membrane of Escherichia coli. Our analysis revealed that, despite its relatively large size, about 10–20 kanamycin molecules per second permeate through OmpF and OmpC under a 10 μM concentration gradient, whereas OmpN does not allow the passage. Molecular simulations elucidate the uptake mechanism of kanamycin through these porins. Whole-cell studies with a defined set of E. coli porin mutants provide evidence that translocation of kanamycin via porins is relevant for antibiotic potency. The values are discussed with respect to other antibiotics.

Type: Article
Title: Kanamycin uptake into Escherichia coli is facilitated by OmpF and OmpC porin channels located in the outer membrane
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acsinfecdis.0c00102
Publisher version: https://doi.org/10.1021/acsinfecdis.0c00102
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: E. coli OmpF OmpC OmpN bacterial porins optimal transport
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10097708
Downloads since deposit
228Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item