UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

3D printed Artificial Cornea for Corneal Stromal Transplantation

Ulag, S; Ilhan, E; Sahin, A; Karademir Yilmaz, B; kalaskar, DM; Ekren, N; Kilic, O; ... Gunduz, O; + view all (2020) 3D printed Artificial Cornea for Corneal Stromal Transplantation. European Polymer Journal , 133 , Article 109744. 10.1016/j.eurpolymj.2020.109744. Green open access

[thumbnail of Kalaskar_1-s2.0-S0014305720301518-main.pdf]
Preview
Text
Kalaskar_1-s2.0-S0014305720301518-main.pdf - Accepted Version

Download (6MB) | Preview

Abstract

The aim of this study is to understand the optical, biocompatible, and mechanical properties of chitosan (CS) and polyvinyl-alcohol (PVA) based corneal stroma constructs using 3D printing process. Corneal stroma is tested for biocompatibility with human adipose tissue-derived mesenchymal stem cells (hASCs). Physico-chemical and chemical characterization of the construct was performed using scanning electron microscopy (SEM), fourier transforms infrared spectroscopy (FTIR). Optical transmittance was analyzed using UV-Spectrophotometer. Results showed fabricated constructs have required shape and size. SEM images showed construct has thickness of 400 µm. The FTIR spectra demonstrated the presence of various predicted peaks. The swelling and degradation studies of 13%(wt)PVA and 13%(wt)PVA/(1, 3, 5)%(wt)CS showed to have high swelling ratios of 7 days and degradation times of 30 days, respectively. The light transmittance values of the fabricated cornea constructs decreased with CS addition slightly. Tensile strength values decreased with increasing CS ratio, but we found to support intraocular pressure (IOP) which ranges from 12 to 22 mm-Hg. Preliminary biostability studies showed that composite constructs were compatible with hASCs even after 30 days’ of degradation, showing potential for these cells to be differentiated to stroma layer in future. This study has implications for the rapid and custom fabrication of various cornea constructs for clinical applications.

Type: Article
Title: 3D printed Artificial Cornea for Corneal Stromal Transplantation
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.eurpolymj.2020.109744
Publisher version: https://doi.org/10.1016/j.eurpolymj.2020.109744
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: https://discovery.ucl.ac.uk/id/eprint/10097176
Downloads since deposit
240Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item