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Inorganic polyphosphate is produced and hydrolysed in F0F1-ATP synthase of mammalian mitochondria

Bayev, AY; Angelova, PR; Abramov, AY; (2020) Inorganic polyphosphate is produced and hydrolysed in F0F1-ATP synthase of mammalian mitochondria. Biochemical Journal , 477 (8) pp. 1515-1524. 10.1042/BCJ20200042. Green open access

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Abstract

Inorganic polyphosphate (polyP) is a polymer present in all living organisms. Although polyP is found to be involved in a variety of functions in cells of higher organisms, the enzyme responsible for polyP production and consumption has not yet been identified. Here we studied the effect of polyP on mitochondrial respiration, oxidative phosphorylation and activity of F0F1-ATPsynthase. We have found that polyP activates mitochondrial respiration which does not coupled with ATP production (V2) but inhibits ADP dependent respiration (V3). Moreover, PolyP can stimulate F0F1-ATPase activity in the presence of ATP and, importantly, can be hydrolysed in this enzyme instead of ATP. Furthermore, PolyP can be produced in mitochondria in the presence of substrates for respiration and phosphate by the F0F1-ATPsynthase. Thus, polyP is an energy molecule in mammalian cells which can be produced and hydrolysed in the mitochondrial F0F1-ATPsynthase.

Type: Article
Title: Inorganic polyphosphate is produced and hydrolysed in F0F1-ATP synthase of mammalian mitochondria
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1042/BCJ20200042
Publisher version: https://doi.org/10.1042/BCJ20200042
Language: English
Additional information: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: F0-F1-ATPase, bioenergetics, inorganic polyphosphates, mitochondria
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences
URI: https://discovery.ucl.ac.uk/id/eprint/10095648
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