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Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440

Salvachúa, D; Werner, AZ; Pardo, I; Michalska, M; Black, BA; Donohoe, BS; Haugen, SJ; ... Beckham, GT; + view all (2020) Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440. Proceedings of the National Academy of Sciences of the United States of America 10.1073/pnas.1921073117. (In press). Green open access

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Abstract

Lignin is an abundant and recalcitrant component of plant cell walls. While lignin degradation in nature is typically attributed to fungi, growing evidence suggests that bacteria also catabolize this complex biopolymer. However, the spatiotemporal mechanisms for lignin catabolism remain unclear. Improved understanding of this biological process would aid in our collective knowledge of both carbon cycling and microbial strategies to valorize lignin to value-added compounds. Here, we examine lignin modifications and the exoproteome of three aromatic–catabolic bacteria: Pseudomonas putida KT2440, Rhodoccocus jostii RHA1, and Amycolatopsis sp. ATCC 39116. P. putida cultivation in lignin-rich media is characterized by an abundant exoproteome that is dynamically and selectively packaged into outer membrane vesicles (OMVs). Interestingly, many enzymes known to exhibit activity toward lignin-derived aromatic compounds are enriched in OMVs from early to late stationary phase, corresponding to the shift from bioavailable carbon to oligomeric lignin as a carbon source. In vivo and in vitro experiments demonstrate that enzymes contained in the OMVs are active and catabolize aromatic compounds. Taken together, this work supports OMV-mediated catabolism of lignin-derived aromatic compounds as an extracellular strategy for nutrient acquisition by soil bacteria and suggests that OMVs could potentially be useful tools for synthetic biology and biotechnological applications.

Type: Article
Title: Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1073/pnas.1921073117
Publisher version: https://doi.org/10.1073/pnas.1921073117
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Pseudomonas putida, biological funneling, extracellular vesicle, lignin valorization, outer membrane vesicle
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Electronic and Electrical Eng
URI: https://discovery.ucl.ac.uk/id/eprint/10095093
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