Knopfel, EB;
Vilches, C;
Camargo, SMR;
Errasti-Murugarren, E;
Staubli, A;
Mayayo, C;
Munier, FL;
... Kloeckener-Gruissem, B; + view all
(2019)
Dysfunctional LAT2 Amino Acid Transporter Is Associated With Cataract in Mouse and Humans.
Frontiers in Physiology
, 10
, Article 688. 10.3389/fphys.2019.00688.
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Abstract
Cataract, the loss of ocular lens transparency, accounts for ∼50% of worldwide blindness and has been associated with water and solute transport dysfunction across lens cellular barriers. We show that neutral amino acid antiporter LAT2 (Slc7a8) and uniporter TAT1 (Slc16a10) are expressed on mouse ciliary epithelium and LAT2 also in lens epithelium. Correspondingly, deletion of LAT2 induced a dramatic decrease in lens essential amino acid levels that was modulated by TAT1 defect. Interestingly, the absence of LAT2 led to increased incidence of cataract in mice, in particular in older females, and a synergistic effect was observed with simultaneous lack of TAT1. Screening SLC7A8 in patients diagnosed with congenital or age-related cataract yielded one homozygous single nucleotide deletion segregating in a family with congenital cataract. Expressed in HeLa cells, this LAT2 mutation did not support amino acid uptake. Heterozygous LAT2 variants were also found in patients with cataract some of which showed a reduced transport function when expressed in HeLa cells. Whether heterozygous LAT2 variants may contribute to the pathology of cataract needs to be further investigated. Overall, our results suggest that defects of amino acid transporter LAT2 are implicated in cataract formation, a situation that may be aggravated by TAT1 defects.
| Type: | Article |
|---|---|
| Title: | Dysfunctional LAT2 Amino Acid Transporter Is Associated With Cataract in Mouse and Humans |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fphys.2019.00688 |
| Publisher version: | http://doi.org/10.3389/fphys.2019.00688 |
| Language: | English |
| Additional information: | © 2019 Knöpfel, Vilches, Camargo, Errasti-Murugarren, Stäubli, Mayayo, Munier, Miroshnikova, Poncet, Junza, Bhattacharya, Prat, Berry, Berger, Heon, Moore, Yanes, Nunes, Palacín, Verrey and Kloeckener-Gruissem. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | amino acid transporters LAT2 and TAT1, gene expression, cataract, ocular tissues, mouse model, patient screen |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10094802 |
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